Journal: Human gene therapy

Article Title: Helper-dependent adenoviral vector achieves prolonged, stable expression of interleukin-10 in rabbit carotid arteries but does not limit early atherogenesis.

doi: 10.1089/hum.2010.175

Figure Lengend Snippet: FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).

Article Snippet: After blocking membranes with 10 mM Tris-HCl buffer, pH 8.0, containing 150 mM sodium chloride, 0.1% Tween 20, and 5% (w/v) nonfat dry milk, IL-10 protein was detected by incubation with goat anti-human IL10 antibodies (R&D Systems, Minneapolis, MN) followed by peroxidase-conjugated donkey anti-goat secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) and detection of bound antibody by chemiluminescence (ImmunStar WesternC kit; Bio-Rad Laboratories, Hercules, CA).

Techniques: In Vitro, Expressing, Plasmid Preparation, Cloning, Virus, Transduction, Western Blot, Marker

Journal: Pathogens

Article Title: An Evaluation of IL-10 Encoded by Cytomegalovirus in the Prediction of Coronary Artery Disease in People Living with HIV

doi: 10.3390/pathogens15020192

Figure Lengend Snippet: Sequence alignments of IL-10 encoded by laboratory CMV strains (2–7), Epstein–Barr Virus (8. EBV), humans (9) and mice (10). The selected cmvIL-10 peptide is included (1) and the perfect match with all CMV strains analysed is highlighted by a red box. Colours denote amino acid variations from the consensus shown in lines 2–7. The alignment was performed using Geneious alignment tool with default parameters. The image was generated using Geneious version 2025.2 created by Biomatters, Auckland, New Zealand < https://www.geneious.com >. Protein sequences were sought from NCBI. Accession numbers are in .

Article Snippet: Binding was detected using 0.2 μg/mL biotinylated polyclonal goat anti-cmvIL-10 antibody (BAF117, R&D Systems) for 2 h. After further washes, 1:2000 streptavidin-conjugated HRP (BD Biosciences, Franklin Lakes, NJ, USA) diluted in 1% BSA/PBS was added for 30 min. Plates were washed and 3,3′-5,5′ tetramethylbenzidine (TMB) substrate (Sigma–Aldrich, St. Louis, MO, USA) was added, reactions were stopped with 1M H 2 SO 4 and read at 450 nm.

Techniques: Sequencing, Virus, Generated

Journal: Journal of Comparative Pathology

Article Title: Cytokine Expression by Macrophages in the Lung of Pigs Infected with the Porcine Reproductive and Respiratory Syndrome Virus

doi: 10.1016/j.jcpa.2009.07.004

Figure Lengend Snippet: Summary of immunohistochemical methodology

Article Snippet: Porcine IL-10 , pAb , Goat serum , RnD Systems , Bouin's , 1 in 20 , Tween.

Techniques: Immunohistochemical staining